KLSCB was established in 2001, based at the Institute of Biochemistry and Cell Biology (IBCB), Shanghai Institutes for Biological Sciences. In order to promote the knowledge and technology integration and collaboration among scientists in stem cell research and medical application, KLSCB was promoted, at the beginning of 2006, to be supervised by SIBS, and the key lab was re-organized so that nine labs from both Institute of Health Sciences (IHS) and IBCB formed the new team, with a new administration office at IHS.

Academic Committee:
Chairman: Gang PEI, Academician, Tong Ji University
Vice-Chairman: Lin LI, SIBS
Member: Ying JIN, Xin WANG, Xuetao PEI, Naihe JING, Yan CHEN, Duanqin PEI, Guoqiang CHEN, Yufang SHI, Huangtian YANG, Qi ZHOU, Xueliang ZHU, Weijun MA, Jianwu DAI

Main Research Focuses:

  1. Establishment of embryonic stem (ES) and induced pluripotent stem (iPS) cell lines and investigation of molecular mechanisms controlling pluripotency and self-renewal;

  2. Differentiation of ES and iPS cells into cardiomyocytes, neural and hematopoietic lineages as well as dissection of underlying mechanisms at a molecular level;

  3. Investigation of signal transduction pathways of differentiation, apoptosis and self-renewal in hematopoietic stem cells using zebrafish as a model system;

  4. Isolation, purification and identification of adult stem cells; Discovery of new cell surface markers of adult stem cells; Application of adult stem cells in disease treatment;

  5. Immuno-associated studies in stem cell transplantation, particularly for the immuno-rejection problem;

  6. Isolation and identification of tumor stem cells to provide new drug targets for tumor treatment. Elucidation of molecular networks regulating tumor development to find new approaches for early diagnosis of tumors.

While the KLSCB re-organized at the beginning of 2006, it has 5 regular member research groups, 1 guest seat research groups, and 1 Stem cell platform. Altogether, KLSCB has now 88 members, including 27 regular scientists and staff and 61 graduate students. The 5 regular member research groups are headed by the following principal investigators:
Yufang SHI, Director, IHS
Ying JIN, IHS
Huangtian YANG, Vice Director, IHS
Tingxi LIU, Director Assistant, IHS
Yanyun ZHANG, Vice Director, IHS

The guest seat research groups are headed by the following principal investigators:
Yiping HU, Professor, Second Military Medical University Shanghai, China

KLSCB focuses its research on stem cell translational research. Some groups continue their efforts in explore the basic scientific truth like the molecular mechanism for the maintenance and regulation of the pluropotency of the embryonic stem cells, high quality stem cell lines establishment, as well as the proliferation, differentiation and apoptosis of stem cells. Others are targeting their research at the solution of the clinical challenge for stem cell application, including the differentiation of stem cells into different types of cell, immunological regulations when stem cells as a cell therapy, and so on. The third group is working on the establishment of core technology platform and training base for stem cell research. Regardless the efforts in different groups, the overall work are guided to the solution of some key problems in stem cell application while the basic mechanism is explored.

Since the reorganization of the KLSCB, significant progress has been made. By the end of June 2006, the member groups of KLSCB already published several papers, which are among the top stem cell research papers generated in China. KLSCB has been also actively participating the activities in the international and domestic stem cell research communities. Following the Eastern China Science and Technology Forum on Stem Cell (Stem Cell Research: From Bench to Bedside) organized by the KLSCB member groups, the professors participated various international conference, symposium, visiting colleagues around the world and deliver lectures in the research institutions around the world. Recently, Dr. Ying Jin has been elected as the annual meeting program committee member of International Society for Stem Cell Research. A China-ISSCR Stem Cell Conference in Shanghai in 2007 is under discussion and actively preparation.

In order to promote stem cell research in Shanghai and China and internationally, KLSCB is working to serve more scientists in their research as a 'public' stem cell research platform. We can currently provide the following service and technology support while continuing efforts to increase our capacity:
        ， Provide human embryonic stem cells (from SHhES1, a hES cell line established in Dr. Jin's lab);
        ， Provide mouse embryonic stem cells (from rES cell lines established in Dr. Jin's lab);
        ， Provide parthenogenetic mouse embryonic stem cells (established in Dr. Jin's lab);
        ， Provide mouse embryonic fibroblast (MEF) cells;
        ， Provide training opportunities at KLSCB in stem cell culture and feeder preparation, etc.
All materials can be provided upon arrangement with MTA.

Contact:
Jun Wu
225 South Chongqing Road, Room 208, Building 1
Shanghai, 200025, China
Tel: 86-21-63847116
Fax: 86-21-63857802
E-mail: Junwu@sibs.ac.cn

Current publications by KLSCB members:

  1. Yanfang Fu, Tingting Du, Mei Dong, Kangyong Zhu, Changbin Jing, Yong Zhang, Lei Wang, Hongbo Fan, Yi Chen, Yi Jin, Guiping Yue, Saijuan Chen, Zhu Chen, Qiuhua Huang, Qing Jing, Min Deng, and Tingxi Liu. Mir-144 selectively regulates embryonic α-hemoglobin synthesis during primitive erythropoiesis. Blood. (2009) 5;113(6):1340-1349.

  2. Zhang Z, Liao B, Xu M, Jin Y. Post-translational modification of POU domain transcription factor Oct-4 by SUMO-1. FASEB J. (2007) 21(12):3042-51.

  3. Li H, Zhang Z, Wang B, Zhang J, Zhao Y, Jin Y. The Wwp2-mediated Ubiquitination of the RNA Polymerase II Large Subunit in Mouse Embryonic Pluripotent Stem Cells. Mol Cell Biol. (2007) 27:5296-5305.

  4. He S, Cao Q, Qiu Y, Mi J, Zhang JZ, Jin M, Ge H, Emerson SG, Zhang Y, Zhang Y. A New Approach to the Blocking of Alloreactive T Cell-Mediated Graft-versus-Host Disease by In Vivo Administration of Anti-CXCR3 Neutralizing Antibody. J Immunol. (2008) 1;181(11):7581-92.

  5. Zhong X, Jin Y. Critical roles of coactivator p300 in mouse embryonic stem cell differentiation and nanog expression. J Biol Chem. (2009) 3;284(14):9168-75.

  6. Yu HM, Wen J, Wang R, Shen WH, Duan S, Yang HT. Critical role of type 2 ryanodine receptor in mediating activity-dependent neurogenesis from embryonic stem cells. Cell Calcium. (2008) 43(5):417-31.